This page provides summary of the data currently available.

We will periodically update the database. We appreciate receiving published offprints, preprints, and personal communications providing C-value estimates for fungi.

Please send DNA C-value data to Dr. Bellis Kullman at the Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, Riia 181, Tartu 51014, Estonia

Fax: + 372 7 383 013
E-mail: fungal-genomesize[at]zbi.ee

Alternatively you can submit C-value data.

Estimation Methods:

• CS = Complete genome Sequencing
• PFGE = Pulsed Field Gel Electrophoresis
• CHEF = Contour clamped Homogeneous Electric Field gel electrophoresis
• FC = Flow Cytometry (Flow Microfluorometry, Flow Microfluorimetry, Fluorescence-Activated Cell Sorting, a cytophotometry technique). Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells or nuclei obtained from a suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake (a cytophotometry technique).
• PI-FC = Flow Cytometry, stained with Propidium Iodide
• DAPI-FC = Flow Cytometry, stained with DAPI
• PC = Photometric Cytometry (classical cytophotometry technique, microscope with a photometer)
• Fe-PC = microspectrophotometry, stained with Feulgen, measuring light absorption
• Fl-PC = microfluorometry (cytofluorometry), stained with Fluorochrome, measuring the light intensities
• IC = Image Cytometry (a cytophotometry technique, an image analysis system which grabs images from the microscope via a digital camera, and calculates intensity or optical density from the grey values of pixels in the nucleus (see Hardie et al., 2002).
• Fe-IC = Image Cytometry, stained with Feulgen, measuring light absorption (also called optical density, OD)
• Fl-IC = Image Cytometry, stained with Fluorochrome, measuring the light intensities
• NS = Not Specified

Cell types:

• FB = Fruit Body
• PC = Pure Culture
• S = Spores
• C = Conidia
• N = intact Nuclei
• V = Various
• NS = Not Specified

Standards and C-value for standards in Mb:

• SC = Saccharomyces cerevisiae, strain YAC M3 (13.10502 Mbp, method CS);
• PO = Pleurotus ostreatus TAA 142824, spore print (25.0 Mbp, with method PI-FC and 24.0 Mbp, with method DAPI-FC, C-values obtained through the primary standard SC (Kullman, 2000));
• TH = Trichophaea hemisphaerioides TFC 97-71 from TAA 147708 (23.3 Mbp, C-value obtained through the secondary standard PO, measurement was performed through intact nuclei with method PI- FC and 22.2 Mbp, C-value obtained through the primary standard SC, measurement was performed through intact cells with method PI-FC (Kullman, 2000));
• ME-7 = Morchella esculenta REG (Weber 7) (25.7 Mbp, with method FlIC). When the absolute DNA content of T. hemisphaerioides, obtained in Kullman (2000), is 23.3 Mbp and the mean value of the same species, as measured in arbitrary units by Weber (1992), is 54.4, then 1 a.u.= 0.43 Mbp. In this case is calculated the absolute values of Morchella esculenta REG (Weber 7) and all other species studied by Weber (1992);
• Glycine max (1.134 pg (Greilhuber and Obermayer, 1997)).

Number of base pairs = mass in pg x 0.978 x 10⁹ (Dolezel et al., 2003).

• 1pg = 978 Mbp
• C-value in Mpb = 978 x C-value in pg
• C-value in pg = C-value in Mbp / 978